ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
The solvent shipping and delivery system has a pump to provide the solvent, that's the cellular period. The mobile phase functions because the carrier of the sample. The pump can deliver solvent within the reservoir to the detector. The pump can pump much more than 50 ml/min of solvent at pressures around 10,000 Pascals.
측정 가능한 농도 범위는 컬럼에 의해서도 결정됩니다. 컬럼 충진제의 종류, 입자 지름, 컬럼의 크기에 따라 분리에 최적인 시료 주입량이 크게 다릅니다.
employs an autosampler to inject samples. As an alternative to using a syringe to push the sample in to the sample loop, the syringe draws sample in the sample loop.
A reversed-section HPLC separation is performed utilizing a mobile period of 60% v/v h2o and 40% v/v methanol. Exactly what is the mobile section’s polarity index?
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The interface in between the HPLC and also the mass spectrometer is technically more challenging than that within a GC–MS due to incompatibility of a liquid cellular section with the mass spectrometer’s high vacuum requirement.
, as an example, has two cellular period reservoirs that happen to be useful for an isocratic elution or simply a gradient elution by drawing solvents from one particular or both of those reservoirs.
The figure below shows the calibration curve and calibration equation with the list of external requirements. Substituting the sample’s peak region into the calibration equation gives the focus of caffeine within the sample as ninety here four.4 mg/L.
With this particular instrument, Every single pump sends its cellular period to your mixing chamber wherever they Incorporate to form the final cell section. The relative speed of The 2 pumps establishes the cellular period’s closing composition.
. Solvent triangle for optimizing a reversed-stage HPLC separation. The three blue circles present cell phases consisting of the natural solvent and drinking water.
It truly is important for laboratory staff to achieve a essential comprehension of HPLC before employing it to analyze compounds precisely and be certain reputable results.
The choice of detector will depend on the precise wants with the Assessment, thinking about components like sensitivity, selectivity, and compatibility With all the cellular section.
The liquid that transports the sample through the column is recognized as the mobile stage. get more info It comprises of a number of solvents chosen determined by the Assessment’s special prerequisites.